Insight into the Catalytic Mechanism of Pseudomonas aeruginosa Exotoxin A

A Novel Insight into Dehydroleucodine Mediated Attenuation of Pseudomonas aeruginosa Virulence Mechanism

Increasing resistance of Pseudomonas aeruginosa (P. aeruginosa) to conventional treatments demands the search for novel therapeutic strategies. In this study, the antimicrobial activity of dehydroleucodine (DhL), a sesquiterpene lactone obtained from Artemisia (A.) douglasiana, was screened against several pathogenic virulence effectors of P. aeruginosa. In vitro, minimum inhibitory concentrati...

The catalytic mechanism of Pseudomonas aeruginosa cd1 nitrite reductase.

The cd1 NiRs (nitrite reductases) are enzymes catalysing the reduction of nitrite to NO (nitric oxide) in the bacterial energy conversion denitrification process. These enzymes contain two distinct redox centres: one covalently bound c-haem, which is reduced by external electron donors, and another peculiar porphyrin, the d1-haem (3,8-dioxo-17-acrylate-porphyrindione), where nitrite is reduced ...

Purification and characterization of Pseudomonas aeruginosa exotoxin.

A trypsin-sensitive, heat-labile exotoxin of Pseudomonas aeruginosa strain P-A-103 has been purified by a procedure that includes membrane ultrafiltration, hydroxylapatite chromatography, ion-exchange cellulose chromatography, and gel filtration chromatography. The procedure resulted in the recovery of 48% of the exotoxin with a 40-fold increase in specific activity (micrograms of protein per m...

Pseudomonas aeruginosa exotoxin: effect on cell cultures.

Induction of neutrophil apoptosis by the Pseudomonas aeruginosa exotoxin pyocyanin: a potential mechanism of persistent infection.

Pseudomonas aeruginosa colonizes and infects human tissues, although the mechanisms by which the organism evades the normal, predominantly neutrophilic, host defenses are unclear. Phenazine products of P. aeruginosa can induce death in Caenorhabditis elegans. We hypothesized that phenazines induce death of human neutrophils, and thus impair neutrophil-mediated bacterial killing. We investigated...